Seq-Balancer: BD Rhapsody™ Dual Index Check (NovaSeqX Leap Chemistry)

Access the web tool here

This tool helps you check and balance dual indexing primers for BD Rhapsody™ kits, specifically for use with NovaSeqX Leap chemistry. It ensures optimal colour balance and compatibility of selected i5 and i7 primers, reducing the risk of sequencing failures due to unbalanced or incompatible index combinations.

Features

• Supports both BD OMICS-One Dual Index Kit and BD OMICS-One Dual Index XT Kit.
• Visualise and analyse colour balance for selected primer combinations.
• Highlights compatible primers based on curated compatibility maps for each kit.
• Designed for use with NovaSeqX Leap chemistry workflows.

How to Use

1. Select Your Indexing Kit
   • Choose either the standard or XT kit using the tabs at the top.
2. Select Primers
   • In each table (i5 and i7), select one or more primers by checking the boxes.
   • The tool will highlight compatible primers and show colour balance analysis for your selection.
3. Review colour Balance
   • The analysis table below shows the colour balance at each base position for your selected primers.
   • Warnings will appear if any position has "No signal" or is unbalanced.
4. Repeat for Each Lane
   • Repeat the process for every lane/sample you plan to run on the NovaSeqX sequencer.

Notes

• This tool is specifically designed for NovaSeqX Leap chemistry workflows.
• Always use the correct compatibility indexes for your kit.
• For best results, select more than one primer to ensure robust colour balance.