Darea (Deep learning-assisted replica image analysis suite) is a software for high-throughput analysis of freeze-fracture replica electron microscopy images.
Install git, Conda (through anaconda/miniconda or miniforge) and Matlab R2018b or later. Download InstallOrUpdateDarea.m (rightclick -> "Save Link As") and place it in an empty folder. Open Matlab, navigate to that folder, rightclick on InstallOrUpdateDarea.m and select "Run". All other dependencies will be installed automatically.
Please refer to the handbook.pdf file for other installation options.
Please refer to the handbook.pdf file for detailed usage instructions
Please cite this software as:
David Kleindienst, Jacqueline Montanaro, Pradeep Bhandari, Matthew J. Case, Yugo Fukazawa and Ryuichi Shigemoto. Deep Learning-Assisted High-Throughput Analysis of Freeze-Fracture Replica Images Applied to Glutamate Receptors and Calcium Channels at Hippocampal Synapses. Int. J. Mol. Sci. 2020, 21, 6737