diff --git a/lib/internal/modules/esm/hooks.js b/lib/internal/modules/esm/hooks.js
index 4571922ed5a0e9..e919310c597595 100644
--- a/lib/internal/modules/esm/hooks.js
+++ b/lib/internal/modules/esm/hooks.js
@@ -646,16 +646,12 @@ class HooksProxy {
 }
 ObjectSetPrototypeOf(HooksProxy.prototype, null);
 
-// TODO(JakobJingleheimer): Remove this when loaders go "stable".
-let globalPreloadWarningWasEmitted = false;
-
 /**
  * A utility function to pluck the hooks from a user-defined loader.
  * @param {import('./loader.js).ModuleExports} exports
  * @returns {ExportedHooks}
  */
 function pluckHooks({
-  globalPreload,
   initialize,
   resolve,
   load,
@@ -671,12 +667,6 @@ function pluckHooks({
 
   if (initialize) {
     acceptedHooks.initialize = initialize;
-  } else if (globalPreload && !globalPreloadWarningWasEmitted) {
-    process.emitWarning(
-      '`globalPreload` has been removed; use `initialize` instead.',
-      'UnsupportedWarning',
-    );
-    globalPreloadWarningWasEmitted = true;
   }
 
   return acceptedHooks;
diff --git a/test/es-module/test-esm-loader-hooks.mjs b/test/es-module/test-esm-loader-hooks.mjs
index 9e25232146d458..8ec1f03019f1fb 100644
--- a/test/es-module/test-esm-loader-hooks.mjs
+++ b/test/es-module/test-esm-loader-hooks.mjs
@@ -446,30 +446,6 @@ describe('Loader hooks', { concurrency: !process.env.TEST_PARALLEL }, () => {
     });
   });
 
-  describe('globalPreload', () => {
-    it('should emit warning', async () => {
-      const { stderr } = await spawnPromisified(execPath, [
-        '--experimental-loader',
-        'data:text/javascript,export function globalPreload(){}',
-        '--experimental-loader',
-        'data:text/javascript,export function globalPreload(){return""}',
-        fixtures.path('empty.js'),
-      ]);
-
-      assert.strictEqual(stderr.match(/`globalPreload` has been removed; use `initialize` instead/g).length, 1);
-    });
-
-    it('should not emit warning when initialize is supplied', async () => {
-      const { stderr } = await spawnPromisified(execPath, [
-        '--experimental-loader',
-        'data:text/javascript,export function globalPreload(){}export function initialize(){}',
-        fixtures.path('empty.js'),
-      ]);
-
-      assert.doesNotMatch(stderr, /`globalPreload` has been removed; use `initialize` instead/);
-    });
-  });
-
   it('should be fine to call `process.removeAllListeners("beforeExit")` from the main thread', async () => {
     const { code, signal, stdout, stderr } = await spawnPromisified(execPath, [
       '--no-warnings',