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Merge pull request #24 from cytomining/update-readme
Update README.md with template for derived repo's README
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README.md

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@@ -7,10 +7,11 @@ The purpose of the repository is to weld together a versioned data processing pi
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**DO THE FOLLOWING AFTER GENERATING A NEW REPO:**
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- Change the title of the README to **Image-based Profiling for [ProjectName]\(project-url\)**.
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- Delete the **Setup** section
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- Keep the notes section, and edit as necessary.
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- Keep the metadata section if you are adopting it, and edit as necessary.
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- Change the title following the format: "Image-based Profiling for [ProjectName]"
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- Fill in all sections below the marker with your project's information
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- Either fill out the metadata template or link to your external tracking system
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- Note that all discussions related to this dataset should happen in the corresponding GitHub issues
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- Delete the instructions (from the top of this document to the marker line below so that the README starts with "Image-based Profiling for [ProjectName]"
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## Setup
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git push
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```
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### Step 4: Fill out experiment information
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----
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This step ensures that relevant experimental information is captured.
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We recommend all users, both internal and external, to diligently track project metadata. For those without a system, a template is provided below.
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**DELETE EVERYTHING ABOVE THIS LINE AND START WITH THE CONTENT BELOW**
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## Metadata template
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# [Project Name]
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Use this section only if you aren't tracking this metadata elsewhere (e.g., the Broad Imaging Platform's has an internal tracker).
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## Overview
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Fill out the whole template on the first batch; for subsequent batches only add what is different.
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[Brief description of the experiment/dataset (2-3 sentences)]
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## Project Information
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- **Start Date**: YYYY_MM_DD (of the first batch)
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- **Related data repos**: [Links to related data repositories]
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- **Metadata Location**: [Link to external metadata tracking system, if applicable]
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- **Analysis Repo**: [Link to associated analysis repositories]
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Note: All discussions related to this dataset should happen in the GitHub issues of this repository.
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## Processing Details
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- **Pipeline Modifications**: [Any changes from standard pipeline]
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- **Other notes**:
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- [Any other notes related to processing]
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## Experimental Metadata
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Below is the template for tracking experimental metadata. Fill this out if not using an external tracking system.
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<details>
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<summary>Metadata Template</summary>
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```
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Cell type : _______ (ex: U2OS)
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Cell source: ________ (ex: Collab lab) (ex: GPP)
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Plate size : _______ (ex: 384)
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Plate brand : _______ (ex: Cell carrier Ultra)
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Cell densit(y/ies) : _______ (ex: 2K/well) (ex: Columns A-D 1K/well, Columns G-H 500/well)
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Type of perturbation : ___________ (ex: Gene overexpression) (ex: CRISPR KO + compounds)
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If (at least partially) genetic:
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If (at least partially) genetic:
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Genetic introduction method : __________ (ex : lentiviral transduction)
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Selection method : _________ (ex: None) (ex: Puromycin 1ug/mL 24 hrs)
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Number of genes : __________ (ex: 384)
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Number of replicates per perturbation : __________ (ex: 5)
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Perturbation time point : _____________ (ex: 72 hrs)
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Staining protocol : ____________ (ex: CellPainting v3 (LINK)) (ex: LipocytePainting (CITATION)) (ex: 1:500 gt anti tubulin (cat #), 1:1000 A488 anti gt (cat #))
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Microscope : ________ (ex: Opera Phenix )
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Mode : ________ (ex: Confocal)
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Microscope : ________ (ex: Opera Phenix )
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Mode : ________ (ex: Confocal)
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Excitation / emission details : ______________ (ex: ex 488 laser, em 550/50; ex 568 laser, em 600/30) (ex: see Index.idx.xml file)
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Objective : _____________ (ex: 20X water 1.0NA)
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Binning : ____________ (ex: 1x1)
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</details>
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## Notes
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## Data Access Instructions
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To download the data in this repo, first, clone this repo
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### Cloning the Repository
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```bash
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git clone [email protected]:<org>/<repo>.git
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```
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then, download the profiles
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### Downloading Data Files
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```bash
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cd <repo>

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