diff --git a/airflow-core/src/airflow/config_templates/config.yml b/airflow-core/src/airflow/config_templates/config.yml
index c4f10ac9624e9..67ae1b62c6cc8 100644
--- a/airflow-core/src/airflow/config_templates/config.yml
+++ b/airflow-core/src/airflow/config_templates/config.yml
@@ -509,6 +509,15 @@ core:
       type: string
       example: ~
       default: ~
+    multi_team:
+      description: |
+        Whether to run the Airflow environment in multi-team mode.
+        In this mode, different teams can have scoped access to their own resources while still sharing the
+        same underlying deployment.
+      version_added: 3.2.0
+      type: boolean
+      example: ~
+      default: "False"
 database:
   description: ~
   options:
diff --git a/airflow-core/src/airflow/dag_processing/bundles/manager.py b/airflow-core/src/airflow/dag_processing/bundles/manager.py
index f13ea96207715..e58b36b272ea0 100644
--- a/airflow-core/src/airflow/dag_processing/bundles/manager.py
+++ b/airflow-core/src/airflow/dag_processing/bundles/manager.py
@@ -193,6 +193,13 @@ def parse_config(self) -> None:
             _add_example_dag_bundle(bundle_config_list)
 
         for bundle_config in bundle_config_list:
+            if bundle_config.team_name and not conf.getboolean("core", "multi_team"):
+                raise AirflowConfigException(
+                    "Section `dag_processor` key `dag_bundle_config_list` "
+                    "cannot have a team name when multi-team mode is disabled."
+                    "To enable multi-team, you need to update section `core` key `multi_team` in your config."
+                )
+
             class_ = import_string(bundle_config.classpath)
             self._bundle_config[bundle_config.name] = _InternalBundleConfig(
                 bundle_class=class_,
diff --git a/airflow-core/tests/unit/dag_processing/bundles/test_dag_bundle_manager.py b/airflow-core/tests/unit/dag_processing/bundles/test_dag_bundle_manager.py
index b1e8b4f8b651a..f911027ef7a79 100644
--- a/airflow-core/tests/unit/dag_processing/bundles/test_dag_bundle_manager.py
+++ b/airflow-core/tests/unit/dag_processing/bundles/test_dag_bundle_manager.py
@@ -57,6 +57,20 @@
             {"my-bundle"},
             id="remove_dags_folder_default_add_bundle",
         ),
+        pytest.param(
+            json.dumps(
+                [
+                    {
+                        "name": "my-bundle",
+                        "classpath": "airflow.dag_processing.bundles.local.LocalDagBundle",
+                        "kwargs": {"path": "/tmp/hihi", "refresh_interval": 1},
+                        "team_name": "test",
+                    }
+                ]
+            ),
+            "cannot have a team name when multi-team mode is disabled.",
+            id="add_bundle_with_team",
+        ),
         pytest.param(
             "[]",
             set(),
diff --git a/airflow-core/tests/unit/dag_processing/test_manager.py b/airflow-core/tests/unit/dag_processing/test_manager.py
index 1b023381bf54f..e3ff30ae07062 100644
--- a/airflow-core/tests/unit/dag_processing/test_manager.py
+++ b/airflow-core/tests/unit/dag_processing/test_manager.py
@@ -1169,6 +1169,7 @@ def test_bundle_names_to_parse(self, bundle_names, expected, configure_dag_bundl
         bundle_names_being_parsed = {b.name for b in manager._dag_bundles}
         assert bundle_names_being_parsed == expected
 
+    @conf_vars({("core", "multi_team"): "true"})
     def test_bundles_with_team(self, session):
         team1_name = "test_team1"
         team2_name = "test_team2"